| FC Alliance 03 Boys Red (TSL2) | FC Alliance (TN) | TN |
| FC Alliance 04B ENPL | FC Alliance (TN) | TN |
| Metro FC B03 HS Rapids | Metro FC (OH) | OH |
| Ohio Celtic Alliance 03 Green | Ohio Galaxies FC | OH |
| Ohio Extreme SC North 03/04 Boys | Ohio Extreme SC | OH |
| One7 Academy 14 | One7 Academy | NC |
| TSC 03 Boys Showcase | Tennessee Soccer Club (TN) | TN |
| Bluegrass SC 02 Boys | Bluegrass SC | KY |
| FC Alliance 02 Boys Red | FC Alliance (TN) | TN |
| FC Alliance 03 Boys ENPL | FC Alliance (TN) | TN |
| LFC 02 Red | Lexington Football Club | KY |
| LNSC 02 Force Black | Lake Norman Soccer Club (LNSC) | NC |
| Metro FC 02 Rapids | Metro FC (OH) | OH |
| Ohio Galaxies FC 02 Red | Ohio Galaxies FC | OH |
| Ohio Galaxies FC 02 White | Ohio Galaxies FC | OH |
| Bluegrass SC 01 Boys | Bluegrass SC | KY |
| Cincinnati United S/m Gremio Boys | Cincinnati United Syc-Mas | OH |
| FC Alliance 01 Boys ENPL | FC Alliance (TN) | TN |
| FC Alliance 01 Boys RED | FC Alliance (TN) | TN |
| FC Alliance 02 Boys ENPL | FC Alliance (TN) | TN |
| LNSC 01 Force Black | Lake Norman Soccer Club (LNSC) | NC |
| Metro FC B01 Rapids | Metro FC (OH) | OH |
| Metro FC North | Metro FC (OH) | OH |
| Metro Rapids 2--Reitsma | Metro FC (OH) | OH |
| One7 01 | One7 Academy | NC |
| Usf Real 01 Boys Premier | USF Real | IN |
| WVFC 01 Black | West Virginia Football Club | WV |
| ACS 00 Orange B | AC Sandhills | NC |
| FC Alliance 00B Red | FC Alliance (TN) | TN |
| Metro FC Boys 00 White | Metro FC (OH) | OH |
| One7 U16 | One7 Academy | NC |
| TSC 2000 Premier | Tennessee Soccer Club (TN) | TN |
| USF Real 00 Boys Premier | USF Real | IN |
| Charlotte Eagles 99 Boys Mutiny | Charlotte Eagles Youth Club | NC |
| FC Alliance 00B ENPL | FC Alliance (TN) | TN |
| FC Alliance 99B Black | FC Alliance (TN) | TN |
| IFC Hammers | Impact FC (NC) | NC |
| Lexington FC 99/00 White | Lexington Football Club | KY |
| One7 00 | One7 Academy | NC |